monolisa hbsag elisa kit  (Bio-Rad)

Journal: Virology Journal

Article Title: Occult Hepatitis B virus infection in previously screened, blood donors in Ile-Ife, Nigeria: implications for blood transfusion and stem cell transplantation

doi: 10.1186/s12985-016-0533-3

Figure Lengend Snippet: Summary of Results. Figure one summarizes the laboratory tests done and the results seen in form of a flow chart. There were 507 subjects and their plasma specimens were screened for HBsAg using ELISA and five were positive. The remaining 502 were then screened for anti-HBc also using ELISA and 354 were found to be positive. The 354 anti-HBc samples were then screened for HBV DNA using real time PCR. HBV DNA was found in 19. (HBV – Hepatitis B virus, HBsAg –Hepatitis B surface Antigen Anti-HBc – antibody to hepatitis B core antigen, ELISA – Enzyme linked immunosorbent assay, PCR – Polymerase chain reaction)

Article Snippet: HBsAg was tested for in all specimens using commercially available Monolisa™ HBsAg ULTRA ELISA kits manufactured by BIORAD (3, bd Raymond Poincare, 92430 Marnes-la-Coquette-France ) with a lower limit of detection estimated to be less than 0.13 IU/ml, according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction